Antiangiogenic activity of photobiomodulation in experimental model using chorioallantoic embryonic membrane of chicken eggs

Antiangiogenic study

Best Red Light Therapy Commentary

8 applications of 670 nm red light therapy had the equivalent effect as bevacizumab therapy.
8 applications of 670 nm red light therapy had the equivalent effect as bevacizumab therapy. (picture sourceOpens in a new tab.)

This study shows that red light therapy can be as effective as bevacizumab, an antiangiogenic treatment for age-related macular degeneration (AMD).

Researchers obtained good vascular results on chicken egg embryonic membranes which have similar vascular growth as seen in AMD.

Eight sessions of red light therapy reduced unwanted vessels in the membrane as efficiently as bevacizumab.

The light therapy reduced vessel length and the number of junctions. Two treatments had no effect; four treatments had some effect; and 8 treatments were as effective as drug therapy.

Red Light Therapy Study

Arq Bras Oftalmol. 2022 Sep 23:S0004-27492022005010217. doi: 10.5935/0004-2749.2021-0524. Online ahead of print.

ABSTRACT

PURPOSE: The purpose of this study was to investigate the vascular effects of photobiomodulation using a light-emitting diode on the chorioallantoic embryonic membrane of chicken eggs grouped into different times of exposure and to detect the morphological changes induced by the light on the vascular network architecture using quantitative metrics.

METHODS: We used a phototherapy device with light-emitting diode (670 nm wavelength) as the source of photobiomodulation. We applied the red light at a distance of 2.5 cm to the surface of the chorioallantoic embryonic membrane of chicken eggs in 2, 4, or 8 sessions for 90 s and analyzed the vascular network architecture using AngioTool software (National Cancer Institute, USA). We treated the negative control group with 50 μl phosphate-buffered-saline (pH 7.4) and the positive control group (Beva) with 50 μl bevacizumab solution (Avastin, Produtos Roche Químicos e Farmacêuticos, S.A., Brazil).

RESULTS: We found a decrease in total vessel length in the Beva group (24.96% ± 12.85%) and in all the groups that received 670 nm red light therapy (2× group, 34.66% ± 8.66%; 4× group, 42.42% ± 5.26%; 8× group, 38.48% ± 6.96%), compared with the negative control group. The fluence of 5.4 J/cm2 in 4 sessions (4×) showed more regular vessels. The number of junctions in the groups that received a higher incidence of 670 nm red light (4× and 8×) significantly decreased (p<0.0001).

CONCLUSION: Photo-biomodulation helps reduce vascularization in chorioallantoic embryonic membrane of chicken eggs and changes in the network architecture. Our results open the possibility of future clinical studies on using this therapy in patients with retinal diseases with neovascular components, especially age-related macular degeneration.

PMID:36169440Opens in a new tab. | DOI:10.5935/0004-2749.2021-0524Opens in a new tab.

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