Diabetes Metab Syndr Obes. 2022 Sep 21;15:2885-2900. doi: 10.2147/DMSO.S374649. eCollection 2022.
PURPOSE: Photobiomodulation (PBM) promotes diabetic wound healing by favoring cell survival and proliferation. This study aimed to investigate the potential of PBM in stimulating cellular migration, viability, and proliferation using the transforming growth factor-Î²1 (TGF-Î²1)/Smad signaling pathway.
METHODS: The study explored the in vitro effects of near infrared (NIR) light on cell viability (survival) and proliferation as well as the presence of TGF-Î²1, phosphorylated TGF-Î² receptor type I (pTGF-Î²R1) and phosphorylated mothers against decapentaplegic-homolog (Smad)-2/3 (p-Smad2/3) in different fibroblast cell models.
RESULTS: Results show a significant increase in cellular migration in wounded models, and increased viability and proliferation in irradiated cells compared to their respective controls. An increase in the presence of TGF-Î²1 in the culture media, a reduction in pTGF-Î²R1 and a slight presence of p-Smad2/3 was observed in the cells.
CONCLUSION: These findings show that PBM at 830 nm using a fluence of 5 J/cm2 could induce cell viability, migration and proliferation to favor successful healing of diabetic wounds. This study contributes to the growing body of knowledge on the molecular and cellular effect of PBM and showcases the suitability of PBM at 830 nm in managing diabetic wounds.
PMID:36172056 | PMC:PMC9510698 | DOI:10.2147/DMSO.S374649