J Biophotonics. 2022 Jul 27:e202200023. doi: 10.1002/jbio.202200023. Online ahead of print.
Visible red light (RL) therapy is a rapidly expanding treatment option for dermatological conditions, including acne, psoriasis, and chronic wounds. It is currently unknown if high fluences of RL induce DNA damage via reactive oxygen species (ROS) stress or other pathways. Our lab previously demonstrated that RL generates ROS in human dermal fibroblasts (HDFs). Other studies show that UV and blue light generate ROS and DNA damage in fibroblasts. This study aims to determine if RL induces DNA damage in HDFs. We found that 320 J/cm2 , 640 J/cm2 , and 1280 J/cm2 RL (633 Â± 6 nm) did not induce measurable DNA damage in the form of cyclobutane pyrimidine dimers (CPD) or 6-4 photoproducts (6-4PP) immediately, three hours, and twenty-four hours following irradiation. Our study further supports that RL therapy is safe and does not induce DNA damage in the form of CPDs or 6-4PPs in human skin fibroblasts. This article is protected by copyright. All rights reserved.
PMID:35894162 | DOI:10.1002/jbio.202200023